Title
Coriander Seed Extract Enhances the Yield of T2 Bacteriophage in E. coli by Stimulating Adenylate Cyclase
Document Type
Article
Publication Date
2018
Publication Title
Research & Reviews: A Journal of Biotechnology
Volume Number
8
Issue Number
2
Abstract
Coriander has been shown to have antibacterial activity in vitro. Relatively low concentrations of coriander seed extract added to LB broth have no effect on the growth of Escherichia coli. The addition of coriander seed extract to E. coli cultures growing in LB broth at a concentration of 0.012% enhanced the yield of T2 bacteriophage up to 225% of the control. Varying the length of time the extract was present in the culture before infection of the bacteriophage showed that the longer the extract was in contact with the bacteria, the greater was the stimulation of T2 bacteriophage yield. Increasing amounts of glucose added to E. coli cultures infected with T2 bacteriophage caused an increase in bacteriophage yield. Increasing the concentration of glucose added to E. coli cultures treated with coriander seed extract which were then infected with T2 bacteriophage caused a decrease in the yield of the bacteriophage. Exposure to coriander seed extract of E. coli cells with a mutation for the catabolite repressor protein or E. coli cells with a mutation for the adenylate cyclase enzyme did not result in an increase of T2 bacteriophage yield as compared to the control. These results suggest that the stimulation of T2 bacteriophage caused by coriander seed extract is due to the stimulation of adenylate cyclase enzyme activity and the production of cAMP within the E. coli cells.
ISSN
2347-7245
First Page
7
Last Page
13
Recommended Citation
Goldstein, Gerald; Bergman, Sarah; Buss, Marielle; Cordes, Hanna; Cranley, Mary; Reed, Nicholas; and Wolfe, Malory, "Coriander Seed Extract Enhances the Yield of T2 Bacteriophage in E. coli by Stimulating Adenylate Cyclase" (2018). Botany & Microbiology Faculty Work. 60.
https://digitalcommons.owu.edu/bomi_pubs/60
Link Out URL
http://techjournals.stmjournals.in/index.php/RRJoBT/article/view/160