Sodium Fluoride Enhances T2 Bacteriophage Yield

Document Type


Publication Date


Publication Title

Research & Reviews: A Journal of Biotechnology

Volume Number


Issue Number



Sodium Fluoride is a strong stimulator of the adenylate cyclase enzyme both in eucaryotic and in procaryotic cells. Stimulation of adenylate cyclase produces cAMP and increases the metabolic activity of bacterial cells by methods that are as yet not completely understood. The addition of either sodium chloride or sodium bromide to bacteriological growth medium moderately affects the growth rate of E. coli cells after 2 h of exposure while the addition of 6 mM sodium fluoride stimulates the growth of E. coli by about 100% but only after 6 h of incubation. After 2.5 h of incubation in the presence of sodium fluoride, there is very little difference in the growth of treated bacterial cells compared to the control. The presence of 42.8 mM sodium chloride in the bacteriological growth medium increases the yield of T2 bacteriophage to 132.6% of the control whereas the presence of 12 mM sodium fluoride or 12 mM sodium bromide increases the yield of T2 bacteriophage to 612% and 678% of the control, respectively. The addition of glucose to bacteriological growth medium enhances the replication of T2 bacteriophage but the addition of increasing amounts of glucose to sodium fluoride treated E. coli cells decreases the sodium fluoride stimulatory effect on T2 bacteriophage replication. These results suggest that the sodium fluoride stimulated production of cAMP increases the metabolic activity of the E. coli cells but has a disproportionate effect on the increase in yield of T2 bacteriophage.



First Page


Last Page


Link Out URL